hi,every professional,
now i wanna pursue a low stringency southern hybridizaion. the probe is ~1kb and with nearly only 70% homology with the target DNA.
how can i decide the hybridization temperature?
thanks.
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#2
fred_33
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Posted 15 March 2005 - 02:03 AM
hi
as you get only 70%homology with your target, i would first try a room temperature assay...
as you get only 70%homology with your target, i would first try a room temperature assay...
#3
lexA
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Posted 15 March 2005 - 03:33 AM
thank you 
but
RT? can the double strand denature and accomplish hybridization successfully in such low temperature?
and do you mean i have to try a temperature grads?
but
RT? can the double strand denature and accomplish hybridization successfully in such low temperature?and do you mean i have to try a temperature grads?
