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southern hybridization temperature


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#1 lexA

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Posted 14 March 2005 - 07:38 PM

hi,every professional,

now i wanna pursue a low stringency southern hybridizaion. the probe is ~1kb and with nearly only 70% homology with the target DNA.
how can i decide the hybridization temperature?

thanks.

#2 fred_33

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Posted 15 March 2005 - 02:03 AM

hi
as you get only 70%homology with your target, i would first try a room temperature assay...

#3 lexA

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Posted 15 March 2005 - 03:33 AM

thank you :unsure:

but :) RT? can the double strand denature and accomplish hybridization successfully in such low temperature?

and do you mean i have to try a temperature grads?




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