for freezing cells i use serul 10%DMSO or serum free medium +10%DMSO and it works very well. It's not necessary to add antibiotics like geneticin or puromycin.
in order to add geneticin or puromycin, should my cell have vector with marker which is resistant to these antibiotica? because the cells will die if it does not have resistant marker, won't it? or the cells are naturally resistant to these?
But if you mean penicilin streptomycin by "antibiotics" you can add it in the medium after thawing. mammalian cells are normally resistant to these classical.
you mean naturally resistant to and not that we have already transfected it with a resistant vector against these antibiotica?
But NEVER add antibiotics during transfection