justwonder, on Mar 14 2005, 11:42 PM, said:
should i add antibiotica :
1. in medium after thawing?
2. in freezing medium?
3. in incubation of transfected cells?
or should i add antibiotica only when my mammalian cells have a vector with the appropriate selectable marker for that antibiotica?
for freezing cells i use serul 10%DMSO or serum free medium +10%DMSO and it works very well. It's not necessary to add antibiotics like geneticin or puromycin.
But if you mean penicilin streptomycin by "antibiotics" you can add it in the medium after thawing. mammalian cells are normally resistant to these classical.
But NEVER add antibiotics during transfection
hope i as helpful.
Edited by fred_33, 15 March 2005 - 01:19 AM.