does anybody have a general protocol for recursive PCR?I will be using 6 oligo @ 100 bp long to make an insert about 500 bplong.
0
1 reply to this topic
#2
anonymous
-
-
- 1,890 posts
Veteran
0
Neutral
Neutral
Posted 23 March 2001 - 10:00 PM
See Prodromou et al Protein Engineering (1992) 8, 827-9
I believe the most important bit is the oligo design, ~20 bases overlap, check sequence of the overlap ends to make sure they don't prime with other oligos. There should be other references if you do a literature search.You can probably contact the guy himself (Chris Prodromou) if you have more question. He has just moved to the Institute of Cancer Research in London.
