designing gene-specific primer for cDNA synthesis
Posted 14 March 2005 - 02:33 AM
Posted 14 March 2005 - 05:59 AM
Posted 14 March 2005 - 11:22 AM
Posted 14 March 2005 - 12:29 PM
If I am not wrong, the gene-specific primer for first strand cDNA synthesis has to be located 3' of your PCR amplicon and antisense to the coding strand, similiar to oligo-dT primer.
To design a gene specific primer for RT, is the primer to the 5 prime of the real time amplicon?
Posted 15 March 2005 - 05:35 AM
If I design separate primers for the RT, can you suggest some conditions to specify in software? Thanks