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Antibiotics selection in mammalian expression


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3 replies to this topic

#1 justwonder

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Posted 13 March 2005 - 08:01 AM

if my vector has an amphicilin and a neomycin selection markers. which one should i use for selection of my mammalian cells?

thanks.

#2 mario2004

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Posted 13 March 2005 - 09:16 AM

I am not sure... but my understanding is that the amp is for selection in bacteria when doing transformation and neo is for selection in mammalian cells when you want to establish stably expressing cells.

cheers!

Mario

#3 justwonder

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Posted 13 March 2005 - 11:56 AM

thanks. :angry:

#4 badcell

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Posted 14 March 2005 - 03:44 AM

That's right. You should use ampicilling for selecting your vector on bacterial cultures, and neomycin/G418 when transfecting mammalian cells.
Cheers!
Science is a wonderful thing if one does not have to earn one's living at it
(A.Einstein)




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