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How to get rid of OCT used in freezing section

Started by tataat2000, Mar 12 2005 11:41 AM

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#1 tataat2000

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Posted 12 March 2005 - 11:41 AM

I have some frozen sections, which were imbedded in OCT. I tried to stain them with HE, using standard protocols, but failed. Is it caused by the OCT? How to get rid of the OCT (It seems that xylene does not work)? Or I have to use paraffin to imbed the sample?

#2 Finnbarr

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Posted 12 March 2005 - 06:22 PM

OCT is very water soluble, so you could try a quick water rinse before hematoxylin, then rinse with water until clear and begin your dehydration up until eosin.

what tissue are you trying to stain?
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