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[Trouble Man]

Hi everybody!
In our lab we tried to synchronize NIH 3T3 cell line by a 18 hour-serum deprivation. The result of our starvation was a 75-80% of cell death after the 18 hours.
Can anybody tell me which is the right way to starvate this cell line? Thanks a lot for your attention!

Trouble Man

[badcell]

That's strange, NIH-3T3 cells can even grow if you throw them on the floor and let them there! When I did cell division experiments culturing the cells with several hormones and factors, my controls in serum-free media didn't die after a 5-days incubation, so 18h incubation shouldn't kill them. Which is the recipe of your serum-free media?