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Serum Starvation


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#1 Trouble Man

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Posted 12 March 2005 - 10:14 AM

Hi everybody!
In our lab we tried to synchronize NIH 3T3 cell line by a 18 hour-serum deprivation. The result of our starvation was a 75-80% of cell death after the 18 hours. :ph34r:
Can anybody tell me which is the right way to starvate this cell line? Thanks a lot for your attention!

Trouble Man

#2 badcell

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Posted 14 March 2005 - 03:29 AM

That's strange, NIH-3T3 cells can even grow if you throw them on the floor and let them there! When I did cell division experiments culturing the cells with several hormones and factors, my controls in serum-free media didn't die after a 5-days incubation, so 18h incubation shouldn't kill them. Which is the recipe of your serum-free media?
Science is a wonderful thing if one does not have to earn one's living at it
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