Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Serum Starvation

  • Please log in to reply
1 reply to this topic

#1 Trouble Man

Trouble Man


  • Members
  • Pip
  • 1 posts

Posted 12 March 2005 - 10:14 AM

Hi everybody!
In our lab we tried to synchronize NIH 3T3 cell line by a 18 hour-serum deprivation. The result of our starvation was a 75-80% of cell death after the 18 hours. :ph34r:
Can anybody tell me which is the right way to starvate this cell line? Thanks a lot for your attention!

Trouble Man

#2 badcell



  • Active Members
  • PipPipPipPipPip
  • 94 posts

Posted 14 March 2005 - 03:29 AM

That's strange, NIH-3T3 cells can even grow if you throw them on the floor and let them there! When I did cell division experiments culturing the cells with several hormones and factors, my controls in serum-free media didn't die after a 5-days incubation, so 18h incubation shouldn't kill them. Which is the recipe of your serum-free media?
Science is a wonderful thing if one does not have to earn one's living at it

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.