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pET expression system

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#1 anonymous



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Posted 04 May 2001 - 09:00 PM

I was used pET29a(+)expression system to expression my interesting protein.I have a trouble. After IPTG induction and than anaysis by SDS-PAGE.I found protein was be expressed but the yield is to low.I want to know how to improve this question!pET29a(+) is use T7 lac promoter,it need final 1mM IPTG induction.I try to understand, if uesd different IPTG concentration to induction maybe can increase expression activity!Thank for your answer!

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