Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Ventation of culture vessles


  • Please log in to reply
5 replies to this topic

#1 justwonder

justwonder

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
0
Neutral

Posted 11 March 2005 - 04:35 AM

how should the lid be when i incubate my cells in:

1. multiwells: should i tilt the lid so that half of the plate is covered and half is exposed directly to the air in the incubator? or should i just have the lid on so the whole plate is covered?

2. culture flask: should the lid be loosened (contamination risk)? or should i tie it completely (my cells can not get air )?

3. dish: should i only cover half of the dish? in this way half is covered and half is exposed directly to the air in the incubator? or should i just have the lid on so the whole dish is covered?

thank you.

#2 chloeo

chloeo

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 11 March 2005 - 07:25 AM

hello
when u incubate your cells in multiwells and dishes you don't need to leave lid at an angle in the incubator as your cells will get infected, air should be able to get in as the seal isn't totally airtight. Generally you only put cells into wells and dishes to do an experiment so they probably won't be in there for ages anyway (use flasks for routine culture).

As for flasks, i guess you don't have vented tops so yes slightly loosen your lids.

hope this helps
:ph34r:

#3 justwonder

justwonder

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
0
Neutral

Posted 11 March 2005 - 11:24 AM

thanks. :ph34r:

#4 justwonder

justwonder

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
0
Neutral

Posted 11 March 2005 - 02:49 PM

BTW what if i have vented tops, should i tie or loosen the lid? :ph34r:

#5 Chantal

Chantal

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 11 March 2005 - 03:37 PM

If you have vented tops, you can tie or loosen the lid. The advantage of the vented top is that you do not need to worry about remembering of opening the cap inside the incubator.

Cells need to be in contact with air inside the incubator otherwise they die. The vented top let air go through, therefore does not matter if you leave the cap tight or not.

For cell culture dishes even if the lid is completely on there is exchange of air anyway, therefore you should leave the lid on.

#6 justwonder

justwonder

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
0
Neutral

Posted 12 March 2005 - 05:16 AM

thanks.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.