Ventation of culture vessles
Posted 11 March 2005 - 04:35 AM
1. multiwells: should i tilt the lid so that half of the plate is covered and half is exposed directly to the air in the incubator? or should i just have the lid on so the whole plate is covered?
2. culture flask: should the lid be loosened (contamination risk)? or should i tie it completely (my cells can not get air )?
3. dish: should i only cover half of the dish? in this way half is covered and half is exposed directly to the air in the incubator? or should i just have the lid on so the whole dish is covered?
Posted 11 March 2005 - 07:25 AM
when u incubate your cells in multiwells and dishes you don't need to leave lid at an angle in the incubator as your cells will get infected, air should be able to get in as the seal isn't totally airtight. Generally you only put cells into wells and dishes to do an experiment so they probably won't be in there for ages anyway (use flasks for routine culture).
As for flasks, i guess you don't have vented tops so yes slightly loosen your lids.
hope this helps
Posted 11 March 2005 - 02:49 PM
Posted 11 March 2005 - 03:37 PM
Cells need to be in contact with air inside the incubator otherwise they die. The vented top let air go through, therefore does not matter if you leave the cap tight or not.
For cell culture dishes even if the lid is completely on there is exchange of air anyway, therefore you should leave the lid on.