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microtiter plate


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#1 justwonder

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Posted 11 March 2005 - 04:01 AM

when i measure the protein concentration using a microtiter plate. should i have the plate covered with its lid when i put it in the microplate reader to measure the OD?

thanks.

#2 jadefalcon

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Posted 11 March 2005 - 07:46 AM

if the lid's diaphanous for the wavelength(s) you use, there should be no problem, since the lid covers all wells (standard as well as sample wells), so there would be a factor, but applied to all wells equally.

I personally prefer to measure ELISA-plates without the lid thogh. gives me a better feeling, but I can't really rationally explain this.:ph34r:

mike

Edited by jadefalcon, 11 March 2005 - 07:47 AM.

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#3 mabusheh

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Posted 15 March 2005 - 09:14 PM

when i measure the protein concentration using a microtiter plate. should i have the plate covered with its lid when i put it in the microplate reader to measure the OD?

thanks.

no lid to measure OD




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