2 replies to this topic

[justwonder]

when i measure the protein concentration using a microtiter plate. should i have the plate covered with its lid when i put it in the microplate reader to measure the OD?

thanks.

[jadefalcon]

if the lid's diaphanous for the wavelength(s) you use, there should be no problem, since the lid covers all wells (standard as well as sample wells), so there would be a factor, but applied to all wells equally.

I personally prefer to measure ELISA-plates without the lid thogh. gives me a better feeling, but I can't really rationally explain this.

mike

Edited by jadefalcon, 11 March 2005 - 07:47 AM.

[mabusheh]

justwonder, on Mar 11 2005, 05:01 AM, said:

when i measure the protein concentration using a microtiter plate. should i have the plate covered with its lid when i put it in the microplate reader to measure the OD?

thanks.

no lid to measure OD