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I'm new to real time PCR


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#1 kbolt

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Posted 10 March 2005 - 01:38 PM

I am designing my primers for real time PCR and I am investigating the best route to take. I checked out TaqMan...seems rather expensive but very user friendly...especially for new-b's like myself.

I may never talk my major professor into the idea of spending the money on TaqMan (since I'm the pioneer of the real time PCR in my lab) so I welcome any help in this area :)

Does anyone have any favorite cost conscience method they like to use? Is there anything less expensive that works similar to TaqMan? Or in the long run does using another method come out to cost about the same?

#2 methylnick

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Posted 10 March 2005 - 10:25 PM

With Taqman, the primers are already optimised for your gene of interest. The low cost way is to use SYBR green and optimise primers yourself. If you can design RT-PCR primers spanning intron exon boundaries you are more likely to develop a workable assy than if you can not. SYBR green if optimised well will work just as well as Taqman. And I stress the "if optimised".

good luck with it!

Nick

All comments and communication are my own personal ones, and are not tied to any of my affiliations. 





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