1 reply to this topic

[andreehung]

Hi all,

I would like to isolate Akt protein, a kinase, from nuclei of cardiomyocytes. Do any of you have experience in this topic ? Please give e.g. a standard protocol for isolation. I should use it at first in western blott.

Thank you for information.

Andree  

[fred_33]

hi
i tink you first have to do total proteins nuclear extract.
You can find a protocol here :

http://www.biochem.n..... Extracts.pdf

here is a receipe for cytoplasmic and nuclear extract :
http://www.protocol-...ump.cgi?ID=1534

finally here the global nuclear extract page from protocol online :
http://www.protocol-...&submit2=Search