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isolation of nuclear protein


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#1 andreehung

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Posted 10 March 2005 - 05:29 AM

Hi all,

I would like to isolate Akt protein, a kinase, from nuclei of cardiomyocytes. Do any of you have experience in this topic ? Please give e.g. a standard protocol for isolation. I should use it at first in western blott.

Thank you for information.

Andree :)

#2 fred_33

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Posted 10 March 2005 - 06:06 AM

hi
i tink you first have to do total proteins nuclear extract.
You can find a protocol here :

http://www.biochem.n..... Extracts.pdf

here is a receipe for cytoplasmic and nuclear extract :
http://www.protocol-...ump.cgi?ID=1534

finally here the global nuclear extract page from protocol online :
http://www.protocol-...&submit2=Search




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