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Dimers on SDS-PAGE


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#1 anonymous

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Posted 08 June 2001 - 09:00 PM

Hello! I'd like to know if it is possible the appearence of two forms of the same protein in SDS-PAGE, the monomer and the dimer, considering that it is a denaturing gel. Thank you.Manuela

#2 anonymous

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Posted 10 June 2001 - 09:00 PM

Probably not on an SDS-PAGE but you cna definitely spot it on a non-denaturing gel.

#3 anonymous

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Posted 10 June 2001 - 09:00 PM

Yes it is possible. Try running the gel under reducing conditins to eliminate the dimer.




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