how to prevent HEK293 cells from coming off the plate
Posted 08 March 2005 - 04:38 PM
Can you give me any suggestion for treating HEK293 cells?
Posted 09 March 2005 - 12:28 AM
maybe you can try to change the plastic type of your plates. i know there are differents types that may increase the attach. But i was wondering, do your cells still dividing themselves?
Posted 09 March 2005 - 02:13 PM
During incubation at 4oC, I guess they are not dividing anymore. However, when I plated them until I used them for experiment, they are still dividing.
Do you know what kind of plastic type of the plate that I can use, right now I am using plate made by polystyrene.
Posted 11 March 2005 - 08:57 AM
i good quite bad news for you. in my lab we use polystyrene too. So the problem may not come from the plastic.
But the bad news is that my colleague just told me that she had worked with 293 cells, actually different from the one i work now with, that were very few adherent on the plate. she says that it depends on the lineage of the 293....
Maybe you can try others 293 in your area.
Sorry for the news.
I wish you good luck.
Posted 14 March 2005 - 03:09 AM