Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

protocol for Immunoprecipitation and 2DE


  • Please log in to reply
2 replies to this topic

#1 Jahrhod

Jahrhod

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 March 2005 - 08:28 AM

hello ! I would like to do an Immuno precipitation and a 2DE but the IP buffer is not compatible with the IEF (strip) . Is it possible to find a buffer for do this ? (I work with membrane proteins)
thanks for your help ...

Edited by Jahrhod, 09 March 2005 - 01:35 AM.


#2 mabusheh

mabusheh

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 35 posts
0
Neutral

Posted 09 March 2005 - 09:36 AM

Hi
Do a buffer exchange using the molecular weight cut off membranes with the IEF buffer you want to use. :rolleyes:

#3 Jahrhod

Jahrhod

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 10 March 2005 - 01:00 AM

thank you for your suggestion




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.