Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

protocol for Immunoprecipitation and 2DE

Started by Jahrhod, Mar 08 2005 08:28 AM

  • Please log in to reply
2 replies to this topic

#1 Jahrhod

Jahrhod

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 March 2005 - 08:28 AM

hello ! I would like to do an Immuno precipitation and  a 2DE but the IP buffer is not compatible with the IEF (strip) . Is it possible to find a buffer for do this ? (I work with membrane proteins)
thanks for your help ...

Edited by Jahrhod, 09 March 2005 - 01:35 AM.

#2 mabusheh

mabusheh

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 35 posts
0
Neutral

Posted 09 March 2005 - 09:36 AM

Hi
Do a buffer exchange using the molecular weight cut off membranes with the IEF buffer you want to use.  :rolleyes:

#3 Jahrhod

Jahrhod

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 10 March 2005 - 01:00 AM

thank you for your suggestion
Back to Protein and Proteomics · Next Unread Topic →


  1. BioForum
  2. → Protocols and Techniques Forums
  3. → Protein and Proteomics
  4. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.