Every time my protein of interest is not expressed!
I really don't know what to do more
HELP ME!!!
Dr. Andrew
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1 reply to this topic
#1
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Posted 08 March 2005 - 08:19 AM
Hi! I'm very sad 'cause I've tried more than once, to infect BT549 cells (breast cancer mesenchymal cell line) with my cDNA in pBabe-puro vector using Nolan's lab protocol and another protocol gave me from another lab.
Every time my protein of interest is not expressed!
I really don't know what to do more
HELP ME!!!
Dr. Andrew
Every time my protein of interest is not expressed!
I really don't know what to do more
HELP ME!!!
Dr. Andrew
#2
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- Active Members
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- 291 posts
Veteran
Posted 08 March 2005 - 09:06 AM
hi
did you make a quantitation of infectivity of your packing-cells-supernatant ?
one of my colleague who works on endothelial cells told me once that it exists cells that are strongly resistant to infctivity. Is there references of infection of your cells? Did you chec at the ATCC about informations?
did you make a quantitation of infectivity of your packing-cells-supernatant ?
one of my colleague who works on endothelial cells told me once that it exists cells that are strongly resistant to infctivity. Is there references of infection of your cells? Did you chec at the ATCC about informations?
