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How can I infect BT549 cells ?


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#1 Dr. Andrew

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Posted 08 March 2005 - 08:19 AM

Hi! I'm very sad 'cause I've tried more than once, to infect BT549 cells (breast cancer mesenchymal cell line) with my cDNA in pBabe-puro vector using Nolan's lab protocol and another protocol gave me from another lab.

Every time my protein of interest is not expressed!

I really don't know what to do more ;)

HELP ME!!!

Dr. Andrew

#2 fred_33

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Posted 08 March 2005 - 09:06 AM

hi
did you make a quantitation of infectivity of your packing-cells-supernatant ?
one of my colleague who works on endothelial cells told me once that it exists cells that are strongly resistant to infctivity. Is there references of infection of your cells? Did you chec at the ATCC about informations?




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