Hi All,
I am optimizing my ChIP sonication settings. After sonication, I did a reverse crosslink, recovered DNA by phenol extraction and ethanol precipitation. Strangely, most settings gave me a smear around 1 kb and a distinct band at 50 bp. What I expected is a smear between 200-1 kb. Should I ignore the 50 bp band?
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#2
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Posted 07 March 2005 - 02:34 PM
kawaka, on Mar 7 2005, 12:13 PM, said:
Strangely, most settings gave me a smear around 1 kb and a distinct band at 50 bp. What I expected is a smear between 200-1 kb. Should I ignore the 50 bp band?
Hi kawaka,
I was wondering what percentage gel you are using, if it is a high percentage I suspect the band you are seeing at 50bp is tRNA or RNA contamination, I think with RNAse this band will dissappear.
Cheers
Nick
#3
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Posted 07 March 2005 - 03:33 PM
Hi Nick, Thank you.
I used 2% gel mistakenly. I never thought of RNA showing up, which I think, makes sense.
K
I used 2% gel mistakenly. I never thought of RNA showing up, which I think, makes sense.
K
#4
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Posted 07 March 2005 - 04:46 PM
kawaka, on Mar 7 2005, 04:33 PM, said:
I used 2% gel mistakenly. I never thought of RNA showing up, which I think, makes sense.
no problemo kawaka
Nick
