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dsOligos annealing buffer


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#1 sungy79

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Posted 04 March 2005 - 08:23 PM

Hi

This is Sung. I am right now working on annealing two complementary single stranded oligos about 60mers. From what I have found, in order to anneal the oligos, I need to use annealing buffer. However, instead of the annealing buffer, does anybody know if I can just use PCR buffer from Roche?

sung

#2 fred_33

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Posted 07 March 2005 - 01:32 AM

uhuh

i did not try this one. So if you do so, please tell us your results.


For information, here is my recipe :
2g of each oligo
Buffer STE (10x) :
tris HCl pH 7,5 100mM
NaCl : 500mM
EDTA pH 8 : 10mM
heat your oligos 5' at 95 and let the temperature slowely decrease to room temp.

Fred

#3 badcell

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Posted 07 March 2005 - 03:53 AM

Hi. I use TE:

2 ug Forward oligo
2 ug Reverse oligo
Up to 50 ul with TE
Heat @ 90C for 3 min
Incubate @37C for 1 h

Cheers!
Science is a wonderful thing if one does not have to earn one's living at it
(A.Einstein)




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