Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Recombinant DNA


  • Please log in to reply
1 reply to this topic

#1 biobimbo

biobimbo

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 March 2005 - 02:57 PM

I am enrolled in a biotech lab 1 course at a local college. For the past 2 weeks we have been conducting experiments on cloning and selection of dna fragments. We used a pUC19 vector and the human follistatin fragment. After all the steps of gel isolating, transformation, digesting and ligation my final gel analysis revealed 2 fragments. The professor said I had a 250bp fragment and another fragment that was a "double insertion".
Why did this occur? Was it during ligation with the ligase where 2 copies of my hfs recombined together? Did it occur afer the digestion with Pst1? I don't know how to make a linear map because I can't figure out what happened. I would appreciate your input!

#2 tfitzwater

tfitzwater

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 96 posts
3
Neutral

Posted 07 March 2005 - 04:31 PM

You need to tell us what restriction enzymes you used to cut the insert and vector so we know what was supposed to happen.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.