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virus vector for transfection


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10 replies to this topic

#1 picomagalhaes

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Posted 03 March 2005 - 08:36 AM

We're looking to transiently transfect gastric epithelial cells with a certain gene but have obtained low efficiency with the promega tfx system. We're now looking for a virus vector system that might offer better efficiency. Can anyone recomend a good (fast, easy, cheap) system?

Thanx!

#2 fred_33

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Posted 07 March 2005 - 01:10 AM

hi

you can use the one mentionned in this topic (pBABE) :
http://www.protocol-...?showtopic=5152

or you can use pLNCX vector, or pSUPER-retro...
(http://www.protocol-...?showtopic=4960)

Fred

#3 picomagalhaes

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Posted 07 March 2005 - 03:36 AM

Thanx!

#4 warsel

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Posted 27 May 2009 - 08:48 AM

I would definitely recommend using a lentiviral system over a retroviral system when dealing with primary cells.

You can also get pretty decent transfections in primary cells (I tried with fibroblasts) with JetPEI transfection reagent.

#5 gfischer

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Posted 27 May 2009 - 10:31 AM

I agree with warsel, lenti is the way to go if you have the facilities to make them, especially if your cells don't divide.
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#6 maha

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Posted 28 May 2009 - 12:14 AM

Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into cells.
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#7 calvin*

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Posted 28 May 2009 - 05:03 AM

Following is the best lentiviral vector(pCSCG) that I have ever used. Gives very good efficiency even in primary epithelial cells. Only disadvantage(?)is it has GFP but no drug election.
AT Addgene
The paper is attached

Attached Files


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#8 catherinecarter85

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Posted 01 June 2009 - 05:17 PM

Hope everything is working out for you Bread :P



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#9 Rupam

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Posted 14 October 2010 - 06:27 PM

SCID-X1 which is FDA approved may be helpful to you.In this retroviruses is used as a vector which completed all the factors you required for your work.

Their is lot more scope in biology to do so many things and to get the knowledge about that scope you can see [url="[url]http://www.wiziq.com/tutorial/22753-5-Scope-of-biology"]scope[/url] of biology[/url].It will surely help you.

Regards
Rupam

#10 lai55

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Posted 08 November 2010 - 06:20 PM

Thanks,,,


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#11 CloneMan

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Posted 15 September 2011 - 05:52 PM

For gene delivery into gastric epithelial cells, adenovirus works much better than lentivirus or retrovirus.




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