10 replies to this topic

[picomagalhaes]

We're looking to transiently transfect gastric epithelial cells with a certain gene but have obtained low efficiency with the promega tfx system. We're now looking for a virus vector system that might offer better efficiency. Can anyone recomend a good (fast, easy, cheap) system?

Thanx!

[fred_33]

hi

you can use the one mentionned in this topic (pBABE) :
http://www.protocol-...?showtopic=5152

or you can use pLNCX vector, or pSUPER-retro...
(http://www.protocol-...?showtopic=4960)

Fred

[picomagalhaes]

Thanx!

[warsel]

I would definitely recommend using a lentiviral system over a retroviral system when dealing with primary cells.

You can also get pretty decent transfections in primary cells (I tried with fibroblasts) with JetPEI transfection reagent.

[gfischer]

I agree with warsel, lenti is the way to go if you have the facilities to make them, especially if your cells don't divide.

[maha]

Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into cells.
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[calvin*]

Following is the best lentiviral vector(pCSCG) that I have ever used. Gives very good efficiency even in primary epithelial cells. Only disadvantage(?)is it has GFP but no drug election.
AT Addgene
The paper is attached

Attached Files

•  Development_of_a_Self_Inactivating_Lentivirus_Vector_Inder_Verma.pdf   675.2KB   516 downloads

[catherinecarter85]

Hope everything is working out for you Bread



pret auto

[Rupam]

SCID-X1 which is FDA approved may be helpful to you.In this retroviruses is used as a vector which completed all the factors you required for your work.

Their is lot more scope in biology to do so many things and to get the knowledge about that scope you can see [url="[url]http://www.wiziq.com/tutorial/22753-5-Scope-of-biology"]scope[/url] of biology[/url].It will surely help you.

Regards
Rupam

[lai55]

Thanks,,,


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[CloneMan]

For gene delivery into gastric epithelial cells, adenovirus works much better than lentivirus or retrovirus.