Expression cloning system for protease expression
Posted 02 March 2005 - 12:36 PM
Please give me your opinions regarding expression cloning system for protease expression ( it has to be extracellular secretion), without affecting the host. Any idea about the use of Pichia expression system?
Thanks in advance.
Posted 02 March 2005 - 08:28 PM
Expression in Bacillus subtilis results in secretion of the protein. You need to append suitable signal peptide ahead of you protein. Expression of such a construct in E.coli results in periplasmic accumulation.
Posted 03 March 2005 - 07:38 AM