Designing primers from amino acid sequence
Posted 02 March 2005 - 08:51 AM
Request for help...
I have partial amino acid sequence of a protein from a snail. I need to clone the corresponding gene from a cDNA library based on primers designed from the aminoacid sequence (backtranslation). Can anyone help me on how to design primers from aminoacid sequence? The probles is that since there are multiple codons for aminoacid, i do not know which one to take.
Posted 03 March 2005 - 04:13 AM
but I'm sure you have already thought of that.
Edited by jadefalcon, 03 March 2005 - 04:16 AM.
Posted 03 March 2005 - 12:33 PM