The system has worked for the past year but has suddenly stopped working. When I put on a PCR run, there is no amplification. I know that its not a problem with the PCR ready mix, cause I am able to amplify other genes with it. I also know that it is not a problem with my primers because I have carried out RT-PCR and there is amplification.
So, I think it may be a problem with the probe (possibly the fluorescent tag). I was just wondering if anyone has had similar problems or has any suggestion as to what is wrong? Also does anybody know of a way to test whether the fluorescent tag is working?
Thanks a mill!
Edited by Emma, 02 March 2005 - 07:00 AM.