siRNA for Akt1
Posted 02 March 2005 - 04:28 AM
I'd like to knock-down Akt1 in my cells, but have no experience in siRNA; therefore I'd like to know whether anyone has successfully used commercial siRNA for Akt, such as those from UBI or from CST ecc...
thank you much for help
Posted 02 March 2005 - 05:58 AM
ambion's database shows that they've developed a siRNA for AKT
You can see the page at :
ozyme has a siRNA too, shipped in a complete kit that includes siRNA, transfection agent, and antibody for the targeted protein
you can see also this topic that discuss of where mRNA should be targeted...
Posted 02 March 2005 - 06:41 AM
Posted 02 March 2005 - 06:58 AM
I wanted to suggest solutions. But i want to point out the fact that for Akt there is VALIDATED siRNA sequences for Akt1 and Akt2.
In general cases of siRNA sold by manufacturers, i did not mean the siRNA products works all, i mean that there is a possibility to find siRNA.
Expresson is right to this point.
Edited by fred_33, 02 March 2005 - 09:19 AM.
Posted 03 March 2005 - 04:19 AM