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Large scale PCR to get milligram of DNA


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#1 wildttv

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Posted 02 March 2005 - 04:01 AM

Hello,
my problem concern the ability to obtain large quantity (about 1 mg) of a 3,8 kb plasmid insert alone (without plasmid) for in vitro assay. I think to use PCR, but How many amplification i'm going to do? are there methods or custom service to overcome this problem?

Thanks

#2 george@CASE

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Posted 02 March 2005 - 11:50 AM

Why not just do a maxiprep from a large bacterial culture and then use restriction enzymes to cut your insert out?




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