Best transfection method/reagent for HeLa cells?
Posted 02 March 2005 - 12:59 AM
Aim is to do some CoIP and localization studies.
I asked different fellows, some say Fugene is the best, some proposed TFX while other said, this is all crap, use Lipofectamine.
Now i'm a little bit puzzled, which one i should buy...
Which transfection method / reagent would you recommend?
Posted 02 March 2005 - 01:19 AM
Posted 02 March 2005 - 01:42 AM
in my lab, extreme gene from roche give the best results.
otherwise, Invitrogen may help you with their products, cause HeLa is a popular cell line. Promega website has a great transfection guide that you can consult online.
Posted 02 March 2005 - 01:47 PM
this is a good question! I worked with lipofectamine, lopofectamine 2000 and several other transfection reagents for my HeLa cells. The one that always worked best for me was Dreamfect from oz biosciences (www.ozbiosciences.com). They say youll reach a transfection efficiency up to 90%, I tried it with a plasmid coding for GFP and then FACSed the cells. I get approx. a transfectionefficiency of 80%, which is more I ever had with Lipofectamine (40%).
It's quite easy to be handeled and you don't need a special transfection medium just DMEM without FCS and Pen/Strep.
All the best
Posted 02 March 2005 - 05:46 PM