Western blot detection other than antibody?
Posted 02 March 2005 - 12:26 AM
Anyone know how to detect and quantify protein in western blot using method other than HRP-antibody? My lab is in tight budget and we are looking for cheaper alternative. Thanx heaps!
Posted 02 March 2005 - 04:06 AM
Posted 03 March 2005 - 01:54 AM
Posted 03 March 2005 - 04:48 AM
Errr, my french is a little bit rusty (i.e. plus mal), and there wasn't much french to begin with, so maybe people here can understand this way better than me, so corrections welcome!
I think margo33 says, you can stain your membrane with coommassie or ponceau and then use comparing methods to determine your protein-concentrations, besides she would use AP-antibodies, too, and wishes you luck!
(I hope this is halfway correct )
Edited by jadefalcon, 03 March 2005 - 04:49 AM.
Posted 03 March 2005 - 05:20 AM
Sorry for the answer in french!
Posted 04 March 2005 - 06:04 AM
I'd say: "swing and a miss", but a close one, sorry!