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Bioanalyser For RNA degradation, contradiction!


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#1 sosea

sosea

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Posted 01 March 2005 - 06:25 PM

By using bioanalyzer 2100 Agilent, I got a strange result. The ratio 28s/18s is 0.9, but seems not so much degradation (basiline is low and not many small peaks between 18s and 28s peak and before 18s peak. The RIN is 8.9 (seems good).

I even used the degradometer to ananlyze the degradation fator, it is no more than 3 (more than 8 means significant degradation happening)

Does anybody know why ratio is so low, but the degradation is not so much? Seems contradiction. B)




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