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real-time PCR/ABI Prism 7700


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#1 microbugger

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Posted 01 March 2005 - 01:41 PM

Can anyone offer any advice as to how to program an ABI Prism 7700 to record a standard curve using Invitrogen LUX primers (FAM-labeled)?

I have followed the protocol on the User Bulletin #2 from Applied Biosystems however I can't retrieve my data; I continue to get an error "Invalid threshold cycle for standard" response once I click the 'analysis' bar.

Any help would be greatly appreciated!

Thanks!

JC

#2 Freiberger

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Posted 17 March 2005 - 05:49 PM

Hi,

I think you can get an update for the software that comes with the SDS from ABI.

Maybe the update will solve your problem...


Good luck!
Freiberger




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