We are seting up the lab for study on methylation causing transcripsional silencing of tumor suppresor genes.
One of the reason of not getting a PCR product in a methylation specific PCR may be improper bisulphite treatment. Otherwise the DNA may not be actually methylated. We are using standard with known methylated CpG island, but we are not getting MSP product .
Can anybody help us how to check the bisulphite treatment is correct or not which we strongly doubt?
Is it anyway possible to check without going for sequencing?
Thanks in advance.
Edited by strepto, 01 March 2005 - 04:13 AM.