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[b4usp]

mRNA is heat sensitive but how RT-PCR is performed to get cDNA  by using mRNA.how musc temparature is maintained in order  to avoid denature mRNA..

[jadefalcon]

the RT reaction is performed (usually) at 42°C which is not harmful to RNA. I know that RNA is hydrolysed in a ion-dependent manner at 70°C, but this temperature is not reached in standard RT reactions. The PCR follwing the RT uses cDNA as template which is heat stable.

mike