20 replies to this topic

[Thapa]

We here grew simply in DMEM  and 10% FBS. They are pretty fine...

[refolder]

I use DMEM-F12 and 10% FCS for my MCF-7 cells, and they love it

[jazmenia]

Hi,,
I grow MCF-7 in DMEM+10% FBS+ antibiotic and they are growing just fine.. they are kind of slow.. so what I do is increase the number of cells, i usually seed around 0.7 million cells in p-25 and they grow to full confulency within a week. you just control the number of cells seeded.

I never change the media the next day, usually do that the third day. and I never added insuline.. and they are growing just fine... and mine grow in epithelila layer form not doms

Edited by jazmenia, 23 November 2009 - 07:31 AM.

[isabellaweng]

jazmenia, on 23 November 2009 - 07:30 AM, said:

Hi,,
I grow MCF-7 in DMEM+10% FBS+ antibiotic and they are growing just fine.. they are kind of slow.. so what I do is increase the number of cells, i usually seed around 0.7 million cells in p-25 and they grow to full confulency within a week. you just control the number of cells seeded.

I never change the media the next day, usually do that the third day. and I never added insuline.. and they are growing just fine... and mine grow in epithelila layer form not doms

I also grow them in RPMI-1640 with 10% FBS, they grow well, the doubling time is silimar to ATCC reported. but when I set up the 96-well plate, treat with various doses of estradiol, they didn'grow well, and there are more and more dead cells,I have no idea what happened to these cells, Dose anyone have any idea? I repeated twice, same thing happened.Can anyone tell me how you do the experiment of MCF-7 cells treated with 17-beta estradiol?

[sushik]

For propagation, I usually grow my MCF-7s in DMEM w/ phenol red, 10% FBS, and 1% pen/strep/glu stock solution.  I only grow them in phenol-red free medium when doing proliferation assays.

I rarely count cells while doing simple passaging, but based on personal experience it seems that the growth rate of MCF-7s is sensitive to cell density.  A flask seeded at 10% confluency might take up to 2 weeks to reach 90% confluency, but a flask seeded at ~20% confluency seems to reach 90% confluency in about 3 days.

[ralfstevens]

Great! I was literally just going to post the same question.

Wuahaha have you actually completed this now? I'm curious to see if everything worked out. I'm back in the lab tomorrow so this will be on top of my list.

When I tried last week I didn't use insulin. Curious to see what happened with you?

I did find a great article on RPMI on Cell Biology a week or so ago. I'd recommend having a look, some great stuff on there. I'll check back later.

Cheers,
Ralf