Posted 28 February 2005 - 08:28 PM
Has anyone experienced anything like this? I'm not sure if this is an annealing temp. problem due to the ladder. Thanks in advance.
Posted 01 March 2005 - 12:52 AM
Is your agarose gel fresh? Another problem could be the size of the well, I have seen with a thick well I get shadows more often.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 01 March 2005 - 06:54 AM
Posted 01 March 2005 - 07:07 AM
Wow, that was difficult to explain. Hope you understand what I mean
Posted 01 March 2005 - 07:29 AM
(P.S you did a good job explaining )
Posted 01 March 2005 - 09:04 AM
i know that a 2% gel is difficult to solubilize (don't know if it's the rght verb... ) make sure your solution is completely dissolved and let dry a longer time.
in the begining of my experiments, i didn't heat enough and it remained part of agarose not solubilized. That provoked shadowing.
Hem, hope i was clear too...