17 replies to this topic

[shane]

MSP has advantages in that you are able to gauge the methylation status by detecting the amplicon. An MSP primer usually ends to discriminate either a methylated or unmethylated C so it is a really quick way to assaying for methylation.

[nazanin kia]

BSP is performed using bisulfite-modified DNA as the template using primers that selectively amplify bisulfite-modified DNA and contain no CpG sites so that both methylated and unmethylated DNA are amplified. The resulting PCR product can then be used in the following three ways:
(1) direct sequencing to examine strand-specific methylation for all population of molecules in the DNA sample;
(2) cloning and sequencing to study the methylation status of individual molecules;
(3) digestion with restriction enzymes to examine methylation of particular CpG sites recognized by the enzymes.

[nazanin kia]

BSP is performed using bisulfite-modified DNA as the template using primers that selectively amplify bisulfite-modified DNA and contain no CpG sites so that both methylated and unmethylated DNA are amplified. The resulting PCR product can then be used in the following three ways:
(1) direct sequencing to examine strand-specific methylation for all population of molecules in the DNA sample;
(2) cloning and sequencing to study the methylation status of individual molecules;
(3) digestion with restriction enzymes to examine methylation of particular CpG sites recognized by the enzymes.