4 replies to this topic

[littlecell]

hello, everyone, would you please recommend me a plasmid preparation kit for transfection? thanks a lot!

[neuromatt]

I use genelute from sigma. Not the cheapest but quick and simple. No messing about with ethanol/isopropanol precipitations at the end. Less steps to go wrong, reliable and good yield

[badcell]

I normally use Qiagen, or Macherey-Nagel which is the same but cheaper. I'm not getting good yields, though. Next time I'll be sure to try this Genelute thing.

[jadefalcon]

Quote

I normally use Qiagen, or Macherey-Nagel which is the same but cheaper. I'm not getting good yields, though.

We use Qiagen or Macherey-Nagel kit, too. but with good results. Qiagen Gigapreps really rule - IMHO.

mike

[littlecell]

THANKS all of your information very much!
I would like to try genelute.

Edited by littlecell, 01 March 2005 - 06:45 AM.