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transfection-grade plasmid


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4 replies to this topic

#1 littlecell

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Posted 28 February 2005 - 02:20 PM

hello, everyone, would you please recommend me a plasmid preparation kit for transfection? thanks a lot!

#2 neuromatt

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Posted 01 March 2005 - 01:43 AM

I use genelute from sigma. Not the cheapest but quick and simple. No messing about with ethanol/isopropanol precipitations at the end. Less steps to go wrong, reliable and good yield

#3 badcell

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Posted 01 March 2005 - 02:49 AM

I normally use Qiagen, or Macherey-Nagel which is the same but cheaper. I'm not getting good yields, though. Next time I'll be sure to try this Genelute thing.
Science is a wonderful thing if one does not have to earn one's living at it
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#4 jadefalcon

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Posted 01 March 2005 - 04:22 AM

I normally use Qiagen, or Macherey-Nagel which is the same but cheaper. I'm not getting good yields, though.


We use Qiagen or Macherey-Nagel kit, too. but with good results. Qiagen Gigapreps really rule - IMHO. :unsure:

mike
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#5 littlecell

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Posted 01 March 2005 - 06:44 AM

THANKS all of your information very much!
I would like to try genelute.

Edited by littlecell, 01 March 2005 - 06:45 AM.





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