My PI and I are thinking of constructing a sage library and we are wondering if it will fit into our budget.
Does anyone in the forum have experience constructing a sage library?
From what I read, making the library would be the cheapest aspect of the project. We would probably buy the invitrogen kit which I guessing is around $500 US <-?
As far as sequencing the clones, I estimate that I would have to sequence about 25 96 well plates.
This works out to ~$3500 for the minipreps (qiagen) and a sequencing cost of $480/ 96 well plate or ~$12,000.
This looks to be about $16,000.
From what I read, free software is available (SAGE2000) to analyze the results.
Is there anything big or small that I would need to concider in planning this experiment?
Ben
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