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Why transformation at lower temp?


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#1 calvin*

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Posted 28 February 2005 - 06:10 AM

I am carrying out KCM transformation. The insert is 8 kb in size. i want to transform it in to DH5alpha/XL1Blue. Is growing the cells at 18 deg C better ? How exactly temp is related to good transformation?
"In my opinion, we don't devote nearly enough scientific research to finding a cure for jerks"-Calvin

#2 seqgirl

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Posted 28 February 2005 - 06:43 AM

I believe the reason for growing at lower temperatures (especially for long inserts) is to prevent recombinants.




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