I am carrying out KCM transformation. The insert is 8 kb in size. i want to transform it in to DH5alpha/XL1Blue. Is growing the cells at 18 deg C better ? How exactly temp is related to good transformation?
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Why transformation at lower temp?
1 reply to this topic
Posted 28 February 2005 - 06:10 AM
"In my opinion, we don't devote nearly enough scientific research to finding a cure for jerks"-Calvin
Posted 28 February 2005 - 06:43 AM
I believe the reason for growing at lower temperatures (especially for long inserts) is to prevent recombinants.