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[bonami_rv]

Hi guys !

   I am wroking on a membrane protein,from baculovirus expressed cells.The protein was in insoluble form and soluble only in the presence of strong  anoinic detegents like SDS,Sarkosyl but not in others.It has enzymatic acticy also.

    How to remove SDS from protein , and confirm my protein is  active with out /less  chage in structure ?

   Apart form Kits which removed detergenys i9s there any way to remove anionic detergent?

[sharath]

SDS , I beleive, can be removed by gel filtration or dialysis.