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PCR or Southern?


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3 replies to this topic

#1 anonymous

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Posted 28 November 2001 - 10:00 PM

PCR is more sensitive of course.

#2 ragno

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Posted 28 November 2001 - 10:00 PM

Hello,I would like to know if PCR is more sensitive than southern blotting about transgene detection.Anyone could tell me?thanksmichel

#3 Heather Neufeld

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Posted 19 January 2002 - 02:30 PM

PCR is more sensitive, but in my experience it can be deceptive because it can give false positive results.  It can take a while to optimise the PCR method for each particular sequence you are looking for in order to reduce these false results.   In my experience a Southern is the only conclusive evidence that I'd be confident with, although doing a PCR on a sample prior to Southern blotting can save a lot of time in selecting out those samples to do the Southern on that are likely positive.  I Hope that helps.

#4 Cthulhu

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Posted 24 January 2002 - 04:20 AM

PCR + Southern hybridization is more sensitive than PCR alone




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