3 replies to this topic

[anonymous]

PCR is more sensitive of course.

[ragno]

Hello,I would like to know if PCR is more sensitive than southern blotting about transgene detection.Anyone could tell me?thanksmichel

[Heather Neufeld]

PCR is more sensitive, but in my experience it can be deceptive because it can give false positive results.  It can take a while to optimise the PCR method for each particular sequence you are looking for in order to reduce these false results.   In my experience a Southern is the only conclusive evidence that I'd be confident with, although doing a PCR on a sample prior to Southern blotting can save a lot of time in selecting out those samples to do the Southern on that are likely positive.  I Hope that helps.

[Cthulhu]

PCR + Southern hybridization is more sensitive than PCR alone