Posted 19 January 2002 - 02:30 PM
PCR is more sensitive, but in my experience it can be deceptive because it can give false positive results. It can take a while to optimise the PCR method for each particular sequence you are looking for in order to reduce these false results. In my experience a Southern is the only conclusive evidence that I'd be confident with, although doing a PCR on a sample prior to Southern blotting can save a lot of time in selecting out those samples to do the Southern on that are likely positive. I Hope that helps.