How big DNA fragment can I clone to pUC19 plasmid?
Posted 25 February 2005 - 02:45 AM
Posted 11 March 2005 - 08:35 AM
Posted 14 March 2005 - 05:54 AM
One very significant factor for large inserts is the method of purification. Using kits based on sodium iodide (e.g. Qiagen) are OK for normal-sized fragments (1-4kb, say) but for really large pieces I recall a phenol-chloroform-based extraction yielded the best results.