Edited by bikashpurohit, 03 March 2005 - 05:38 AM.
bacterial culture contamination
Posted 24 February 2005 - 09:32 AM
the advice I can give you is too keep your ampi-plates not for too long, since then fungal groth can occur, and inoculate your autoclaved medium with fresh bacterial colonies. when in doubt, just transfer the colonies to a fresh plate, incubate overnight and use fresh colonies of that plate to inoculate you liquid culture. this costs you a day max, but if your cultures are contaminated, there are two or more days of work lost....
Posted 10 June 2009 - 02:19 AM
Posted 10 June 2009 - 01:26 PM
If this is from a natural source - you will have bacterial and (other) fungal contaminants and probably mites as well. In any case, it appears you're not working with a pure culture. You might try to cut out some agar plugs with apical (end) tips of the growing fungus and reinoculate those separately.