I am having real trouble getting a couple of ligations to work and have been searching around for any protocols that may help me out. I have come across a protocol for in gel ligation, where the RE digested insert and vector are run on a low melting point gel, the fragments are excised and melted and used directly in the ligation reaction
Has anyone used this method before? Does it work? Are there any special considerations such as liagse type i have to think about? Does this method work better for troubled ligations than the standard method?
Cheers for any help
Edited by sandie, 23 February 2005 - 06:15 PM.