elution sample form Mono Q column
Posted 22 February 2005 - 08:35 PM
I loaded my sample; halophilic proteinase contained high negative charge in the surface of molecules, in Mono Q column. I could not eluted the sample form column, eventhough 4M NaCl in 50 mM Tris-HCl, pH 8.0 + 1 mM CaCl2, were used. Please introduced me, what is the suitable buffer for eluted sample
Thank everybody for the good recommentation.
Posted 22 February 2005 - 08:58 PM
Posted 23 February 2005 - 03:21 AM
Posted 23 February 2005 - 12:42 PM
Edited by ZDF, 23 February 2005 - 12:43 PM.
Posted 24 February 2005 - 08:07 PM
Natioanl Chemical Laboratory,