Dose anyone know the marker for the exocytosis vesicles? I want to use it for the detection with fluorescence probing and subsequent confocal microscopy.
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fred_33
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Posted 23 February 2005 - 02:36 AM
hi
you can find specific markers for exocytosis. here is some :
-insulin
-norepinephrin
-GABA
-glucagon (don't know if it's the same word in english)
-growth fators
but the question is what type of vesicle? cell type, regulated or constitutive exocytosis...
You can either find general markers for regulated exocytosis : SNARE proteins (syntaxin, SNAP 25, VAMP) but each one has many isoforms...
may you tell us more?
Hope that helps.
Fred
you can find specific markers for exocytosis. here is some :
-insulin
-norepinephrin
-GABA
-glucagon (don't know if it's the same word in english)
-growth fators
but the question is what type of vesicle? cell type, regulated or constitutive exocytosis...
You can either find general markers for regulated exocytosis : SNARE proteins (syntaxin, SNAP 25, VAMP) but each one has many isoforms...
may you tell us more?
Hope that helps.
Fred
