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BAC cloning problems


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#1 Dragan

Dragan

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Posted 18 February 2005 - 03:07 PM

Hello,
I am trying to clone viral genomic DNA into BAC vector (pCCBACI by Epicentre).
I had no success so far, although I followed their protocol to the word.
I have digested my viral genomic DNA with HindIII and used the appropriate kit (Copy Control HindIII) but I could not even get a single colony on my plates.
I have tried everything, from slightly changing ligation conditions to re-circularizing the vector and cutting it with my fresh HindIII enzyme.

If there's anyone out there using the same kit with some useful advice, please let me know!!!


Best regards,

Dragan




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