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BAC cloning problems

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#1 Dragan



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Posted 18 February 2005 - 03:07 PM

I am trying to clone viral genomic DNA into BAC vector (pCCBACI by Epicentre).
I had no success so far, although I followed their protocol to the word.
I have digested my viral genomic DNA with HindIII and used the appropriate kit (Copy Control HindIII) but I could not even get a single colony on my plates.
I have tried everything, from slightly changing ligation conditions to re-circularizing the vector and cutting it with my fresh HindIII enzyme.

If there's anyone out there using the same kit with some useful advice, please let me know!!!

Best regards,


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