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Loop sequences


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#1 ashwin raut

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Posted 18 February 2005 - 01:27 PM

Can anybody suggest best loop sequence for my small hairpin RNA ?
ashwin

#2 expresson_help

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Posted 21 February 2005 - 03:44 AM

Ah loop sequences - the holy grail of shRNA design... I'm afraid different loop sequences work differently with different shRNAs. For the sequence we recommend and to generate your sequences goto
http://www.expresson...agenerator.html

#3 fred_33

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Posted 21 February 2005 - 03:50 AM

hi
you can see the sequence from brummelkamp et al.Science 296, 550-3
you can see the paper of denti et al Mol Ther. 2004 Jul;10(1):191-9.

I first try the one from brummelkap and al and recently chaged for the one from denti and al. without changing my siRNA sequence. That works correctly in both cases.

Fred




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