I am newcomer of this forum. I met problems in doing chip assay.I have two pairs of primers to amplify my target DNA(different length, 360bp and 120bp respectively). Use one pairs of primers i got one correct band(360 bp) in Input DNA, but didn't got it in the other groups(anti--protein of interest, Normal IgG and No Ab), when use another pairs of primers , I detected the expected band(120 bp) in all groups(input , anti-protein of interest, Normal IgG and No Ab groups).
I used Active Motif chip kit for chip assay.Did anyone use this kit and meet same problems?
Any suggestion will be welcome.
non-specific band in ChIP assay
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