non-specific band in ChIP assay

Started by hongwei wang, Feb 18 2005 08:39 AM

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#1 hongwei wang

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Posted 18 February 2005 - 08:39 AM

Hi All,

I am newcomer of this forum. I met problems in doing chip assay.I have two pairs of primers to amplify my target DNA(different length, 360bp and 120bp respectively). Use one pairs of primers i got one correct band(360 bp) in Input DNA, but didn't got it in the other groups(anti--protein of interest, Normal IgG and No Ab), when use another pairs of primers , I detected the expected band(120 bp) in all groups(input , anti-protein of interest, Normal IgG and No Ab groups).

I used Active Motif chip kit for chip assay.Did anyone use this kit and meet same problems?

Any suggestion will be welcome.