7 replies to this topic

[Joana]

Hey all!

Small question for protein people.  I want to use a nontoxic buffer with pH  5-6 to my proteins, which buffer you recommend?
Could just PBS be used or something?

Thanks.

[Simonsays]

Depends on what you want to do... adjusted PBS or HBSS would seem fine to me... or some Tris based buffer.

Simon

[bigworm]

Simonsays, on Feb 17 2005, 02:14 PM, said:

Depends on what you want to do... adjusted PBS or HBSS would seem fine to me... or some Tris based buffer.

Simon

Can Tris based buffer be adjusted to pH 5~6?

[Simonsays]

Quote

Can Tris based buffer be adjusted to pH 5~6?

I frankly don't know!?!? The more acidic Tris solution we use is 6.8...
Simon

[sharath]

tris cannot be used at ph less than 7.5. its buffering capacity diminishes below that pH. It wont buffer at all in the range 5-6. The buffer capacity of can system depend on the pka of the compounds used. for tris it is 8.3

Sharath.B
research Fellow
National Chemical Laboratory
India

[mabusheh]

PBS is fine I guess

[sharath]

A good choice for pH 5-6 is citrate-phospahte buffer

[leekaming]

If it is 5, i suggest acetate buffer
If it is 6, i suggest phosphate buffer